Te was produced in the pEPKanS template using extended oligonucleotides (Ultramers

Te was produced in the pEPKanS template applying extended oligonucleotides (Ultramers; Integrated DNA Technologies , Coralville, IA) RRV75STOPsKanS_FP (TCCTCTTCTGGACTAAGGGCTGCAGCGG CCCAGCGAGGGTTCGTGCGTTAGGCCATATTTGCTAAGTCTAGC CGAGGATGACGACGATAAGTAGGG) and RRV75STOPasKanS_RP (AAAGTCCCACCCGCTTGCTTTTGCGCCGGCTAGACTTAGCAAA TATGGCCTAACGCACGAACCCTCGCTGGGCCCAACCAATTAACC AATTCTGATTAG) (where the bold and…