70 1013 954 970 970 979 979 1022 978 1022 1022 979 979 979 979 979 979 1022 979 979 978 978 979 979 -1.89(0.72) -0.98 (0.5) 3.71(0.95) 2.28 (0.72) two.35(0.77) 1.27 (0.55) 1.63(0.55) 1.04 (0.43) 1.55(0.57) 0.97 (0.44) two.07(0.68) 0.86 (0.42) -2.03(0.76) -1.53(0.58) 2.22(0.81) 1.07 (0.54) 1.six(0.6) 0.82 (0.42) -0.89 (0.45) -1.eight(0.69) 1.95(0.63) 1.14 (0.46) 2.06(0.67) 1.13 (0.48) two.49(0.77) 1.35 (0.55) 1.16 (0.59) -2.64(0.69) -0.99 (0.41)2.121 three.88 six.81 9.62 6.04 9.48 5.58 10.44 6.07 3.86 14.81 five.89 -2.504 6.89 7.54 three.86 7.06 three.9 -1.607 7.09 1.556 1.417 8.68 5.95 7.31 four.9 9.2 4.09 –

70 1013 954 970 970 979 979 1022 978 1022 1022 979 979 979 979 979 979 1022 979 979 978 978 979 979 -1.89(0.72) -0.98 (0.five) three.71(0.95) 2.28 (0.72) 2.35(0.77) 1.27 (0.55) 1.63(0.55) 1.04 (0.43) 1.55(0.57) 0.97 (0.44) 2.07(0.68) 0.86 (0.42) -2.03(0.76) -1.53(0.58) two.22(0.81) 1.07 (0.54) 1.six(0.6) 0.82 (0.42) -0.89 (0.45) -1.eight(0.69) 1.95(0.63) 1.14 (0.46) two.06(0.67) 1.13 (0.48) 2.49(0.77) 1.35 (0.55) 1.16 (0.59) -2.64(0.69) -0.99 (0.41)2.121 3.88 6.81 9.62 6.04 9.48 five.58 10.44 6.07 three.86 14.81 five.89 -2.504 six.89 7.54 3.86 7.06 three.9 -1.607 7.09 1.556 1.417 eight.68 5.95 7.31 4.9 9.two four.09 -1.781 6.94 three.86 15.3 10.11 9.27 five.0.00687 0.00958 0.00909 0.00193 0.00122 0.00208 0.00188 0.00123 0.00119 0.0097 0.00012 0.00141 0.0047 0.00866 0.00603 0.00974 0.00787 0.00938 0.00152 0.00776 0.00996 0.0044 0.00321 0.00133 0.00687 0.00357 0.00242 0.00775 0.00352 0.00841 0.00974 0.00009 0.00003 0.00232 0.00232 ** *** * * ** * * * * * *solute carrier family members 25 (mitochondrial carrier phosphate carrier) member 25 ptc7 protein phosphatase homolog (cerevisiae) ptc7 protein phosphatase homolog (cerevisiae)tbt-binding protein tbt-binding protein solute carrier household memberglycerol-3-phosphate dehydrogenase glycerol-3-phosphate dehydrogenase novel protein vertebrate stabilinvacuolar protein sorting 4b kiaa1219 proteintuberous sclerosis 1 dipeptidyl peptidase 7 dipeptidyl peptidase 7 loc795887 protein loc795887 protein novel protein novel proteinRobinson et al. BMC Genomics 2014, 15:541 http://biomedcentral/1471-2164/15/Page 7 ofTable four Suggestive and important QTL for trait hours of survival soon after challenge using a. hydrophila detected employing PLINK (QFAM) and GenAbel (FASTA and GRAMMA) analyses in 21 L. rohita households (Continued)23 24 24 25 27.4 55.9 55.9 46.1 64731_210 67606_298 67606_298 54056_576 QFAM FASTA GRAMMA FASTA 1022 979 979 979 -3.63(1.14) -2.07 (0.83) 1.7(0.65) 1.41 ten.21 6.25 6.87 0.00308 0.0014 0.00101 0.00876 * * serine lengthy chain base subunit 1 serine extended chain base subunit 1 n-ethylmaleimide sensitive fusion protein attachment protein alphaLG, linkage group; Pos, location on LG in centimorgans; N, number of progeny and parents analysed; Effect, allele substitution effect from the minor allele with common error in parenthesis (FASTA and GRAMMAS); Stat, test statistic linear regression coefficient for QFAM, chi-square with a single degree of freedom for FASTA and GRAMMA analyses; P, point-wise empirical P-value (QFAM) or permuted P-value with one particular degree of freedom corrected for inflation factor lambda (FASTA and GRAMMA); Sig, significance following Bonferroni correction (*, P 0.4-Nitrobutan-1-ol Chemscene 05; **, P 0.2-(Pyrrolidin-3-yl)acetic acid Chemscene 01; ***, P 0.PMID:23514335 001). GeneID, closest SNP homology from BLAST. Tests were viewed as suggestive when P 0.01 prior to Bonferroni correction.has suggestive associations with each traits (Tables 4, 5 and six). SNP 16321_60 with homology for the integrin alpha fg-gap repeat maps to 48 cM on LG2, within 1 cM of 3 SNPs with suggestive associations on hours of survival (Additional file 1, Table 4). SNP 134389_297 with homology to lymphocyte-specific protein tyrosine kinase maps roughly two cM from SNP 89585_200 (suggestive association with dead or alive) on LG3 (Additional file 1, Tables 5 and six). SNP 98520_125 with homology to proteasome subunit beta type-6 precursor maps towards the very same position, 9.6 cM along LG5, as SNP 4797_109 (suggestive association with hours of survival) (More file 1, Tables 4 and 6). SNPs 111876_59 and 53025_556 with homology to the key histocompatibil.