Product Name :
NCK1 Recombinant Rabbit Monoclonal Antibody [JU41-80]
Predicted band size :
43 kDa
Observed band size :
Synonyms:
Cytoplasmic protein NCK1 antibody Melanoma Nck protein antibody MGC12668 antibody NCK 1 antibody NCK adaptor protein 1 antibody NCK alpha antibody NCK antibody NCK tyrosine kinase antibody Nck-1 antibody NCK1 antibody 展开
Function :
The NCK family of SH2/SH3 adaptor proteins consists of two members, NCK1 (NCKα) and NCK2 (NCKβ), which couple tyrosine kinase signaling, including the EGF and PDGF receptor-pathways, to downstream signaling proteins.1,3-Cyclopentanedione Chemical name Specifically, overexpression of Nck1 in NIH/3T3 cells decreases DNA synthesis stimulated by EGF. Furthermore, the SH2 domain of NCK2 inhibits EGF- and PDGF-induced DNA synthesis. The SH3 domain of NCK binds a proline-rich domain on PAK, a known actin cytoskeleton regulator.Price of 1-(6-Bromopyridin-3-yl)piperazine The NCK protein thus mediates the interaction between PAK and RAC.PMID:33596797 The NCK2 protein binds human PDGFR-b (Tyr 1009); overexpression of Nck2 inhibits PDGF-induced membrane ruffling and lamellipod formation. Various growth factor receptors, cell surface antigens and adhesion molecules phosphorylate mammalian NCK1 and NCK2. The human NCK1 and NCK2 genes map to chromosomes 3q22.3 and 2q12.2, respectively.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within Human NCK1 aa 204-296 / 377.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Coupling :
unconjugated
Clone number:
JU41-80
RRID:
AB_3070677
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
43 kDa
Positive control :
Hela cell lysate, NIH/3T3 cell lysate, rat kidney tissue lysate, mouse testis tissue lysate, LOVO, A431, HUVEC, mouse testis tissue, rat testis tissue, human tonsil tissue, human kidney tissue, Jurkat.